Basic methods in protein purification and analysis : a laboratory manual / edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis.
Material type:
TextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2009.Description: vii, 436 p. : ill. ; 29 cmISBN: - 9780879698683 (hardcover : alk. paper)
- 9780879698676 (pbk. : alk. paper)
- Proteins -- Purification -- Laboratory manuals
- Proteins -- Analysis -- Laboratory manuals
- Proteins -- isolation & purification -- Laboratory Manuals
- Chromatography -- methods -- Laboratory Manuals
- Electrophoresis -- methods -- Laboratory Manuals
- Immunoprecipitation -- methods -- Laboratory Manuals
- Proteins -- analysis -- Laboratory Manuals
- 572/.6078 22 SIM/B
- QP551 .B265 2009
- 2008 M-826
- QU 25
| Item type | Current library | Home library | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
Technical Reference Book
|
Central Library, IIT Bhubaneswar | Central Library, IIT Bhubaneswar | 572/.6078 SIM/B (Browse shelf(Opens below)) | Available | 7855 |
Includes bibliographical references and index.
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
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